Keyword Analysis & Research: pvui
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PvuI | NEB
https://www.neb.com/en/products/r0150-pvui
WEBProduct Information. PvuI has a High Fidelity version PvuI-HF ® ( NEB #R3150 ). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
DA: 61 PA: 88 MOZ Rank: 80
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PvuI-HF® | NEB
https://www.neb.com/en/products/r3150-pvui-hf
WEBAn E. coli strain that carries the cloned and modified PvuI gene from Proteus vulgaris (ATCC 13315). This product is related to the following categories: Restriction Endonucleases P R Products, High-Fidelity (HF®) Restriction Endonucleases Products, Time-Saver Qualified Restriction Enzymes Products
DA: 48 PA: 19 MOZ Rank: 51
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PvuI (10 U/μL) - Thermo Fisher Scientific
https://www.thermofisher.com/order/catalog/product/ER0621
WEBFeatures. • Superior quality—stringent quality control and industry leading manufacturing process. • Convenient color-coded Five Buffer System. • Includes universal Tango buffer for double-digestions. • BSA premixed in reaction buffers. • Wide selection of restriction endonuclease specificities. Applications. • Molecular cloning.
DA: 16 PA: 75 MOZ Rank: 81
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PvuII | NEB
https://www.neb.com/en/products/r0151-pvuii
WEBProduct Information. PvuII has a High Fidelity version PvuII-HF ® ( NEB #R3151 ). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
DA: 71 PA: 26 MOZ Rank: 8
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PvuI - Promega Corporation
https://www.promega.com/products/cloning-and-dna-markers/restriction-enzymes/pvui/
WEBThe PvuI site is regenerated in the ligation product, but the SgfI site is not. Incubation Conditions: Buffer D. 37°C. Source: Proteus vulgaris. Storage Buffer: 10mM Tris-HCl (pH 7.3 @ 25°C), 50mM NaCl, 0.1% Triton® X-100, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol. Percent Activity in 4-CORE® Buffer: Frequency of Cutting:
DA: 45 PA: 66 MOZ Rank: 60
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FastDigest PvuI - Thermo Fisher Scientific
https://www.thermofisher.com/order/catalog/product/FD0624
WEBThermo Scientific FastDigest PvuI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers. The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up ...
DA: 45 PA: 65 MOZ Rank: 55
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PvuI | NEB
https://www.neb.ca/detail.php?p=r0150-pvui
WEBProperties & Usage. Unit Definition. One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Reaction Conditions. 1X NEBuffer™ r3.1. Incubate at 37°C. 1X NEBuffer™ r3.1. 100 mM NaCl. 50 mM Tris-HCl. 10 mM MgCl 2. 100 µg/ml Recombinant Albumin. (pH 7.9 @ 25°C)
DA: 94 PA: 17 MOZ Rank: 57
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PvuI-HF® | NEB
https://www.neb.ca/detail.php?p=r3150-pvui-hf
WEBHigh-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer ( rCutSmart ™ Buffer ). All HF-restriction enzymes come with Gel Loading Dye, Purple (6X). Enjoy the enhanced performance and added value of our engineered ...
DA: 47 PA: 14 MOZ Rank: 89
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Cloning and characterization of genes for the PvuI restriction and
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC334411/
WEBJan 1, 2001 · Abstract. The genes encoding the endonuclease and the methylase of the PvuI restriction and modification system were cloned in E.coli and characterized. The genes were adjacent in tandem orientation spanning a distance of 2200 bases. The PvuI endonuclease was a single polypeptide with a calculated molecular weight of 27,950 …
DA: 40 PA: 60 MOZ Rank: 76
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Product Information: PvuI , #ER0621 - Thermo Fisher Scientific
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0012162_PvuI_10_UuL_300U_UG.pdf
WEBPvuI is supplied in: 10 mM Tris-HCl (pH 7.5 at 25°C), 300 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA and 50% glycerol. Recommended Protocol for Digestion • Add: nuclease-free water 16 µL 10X Buffer R 2 µL DNA (0.5-1 µg/µL) 1 µL PvuI 0.5-2 µL • Mix gently and spin down for a few seconds. • Incubate at 37°C for 1-16 hours.
DA: 42 PA: 92 MOZ Rank: 44