Keyword Analysis & Research: photobleaching
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Photobleaching - Wikipedia
https://en.wikipedia.org/wiki/Photobleaching
Web ResultIn optics, photobleaching (sometimes termed fading) is the photochemical alteration of a dye or a fluorophore molecule such that it is permanently unable to fluoresce. This is caused by cleaving of covalent bonds or non-specific reactions between the fluorophore and surrounding molecules.
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Photobleaching Principles | Thermo Fisher Scientific - US
https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-analysis-learning-center/molecular-probes-school-of-fluorescence/fluorescence-basics/fluorescence-fundamentals/photobleaching-principles.html
Web ResultWhat is photobleaching? A fluorophore can repeatedly undergo the fluorescence process—in theory, indefinitely. This is extremely useful, because it means that one fluorophore molecule can generate a signal multiple times.
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Photobleaching - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/chemistry/photobleaching
Web ResultPhotobleaching —the irreversible photochemical destruction of a fluorophore—leads to two kinds of artifacts on different timescales. (1)The sudden disappearance of a fluorophore results in shorter residence times in the detection volume and apparent faster diffusion.
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Photobleaching in Fluorescence Microscopy - News-Medical.net
https://www.azolifesciences.com/article/Photobleaching-in-Fluorescence-Microscopy.aspx
Web ResultFeb 1, 2021 · Photobleaching is the phenomenon when a fluorophore loses its fluorescence due to damage induced by light. This leads to loss of fluorescence and signal while imaging a sample. Real fluorescence microscopic view of human neuroblastoma cells. Image Credit: Vshivkova / Shutterstock.com.
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Photobleaching in Fluorescence Imaging - Thermo Fisher Scientific
https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-analysis-learning-center/molecular-probes-school-of-fluorescence/imaging-basics/protocols-troubleshooting/troubleshooting/photobleaching.html
Web ResultPhotobleaching of your fluorescence signal during imaging can occur with a variety of fluorophores. The photochemical destruction of the fluorophore is observed as a fading of the fluorescence signal while you are doing your imaging experiment.
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Photobleaching - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/photobleaching
Web ResultPhotobleaching of either the donor or acceptor molecules can be utilized to detect the effects of FRET on the kinetics of the fluorescence of either. Photobleaching is the process whereby a fluorophore is converted to a non-fluorescent species, for instance in the presence of oxygen.
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FRAP and Other Photobleaching Methods | SpringerLink
https://link.springer.com/referenceworkentry/10.1007/3-540-29623-9_4790
Web ResultPhotobleaching can reveal a protein's diffusional properties, movement between compartments within cells and life history. Photobleaching techniques depend on the availability of fluorescently labeled molecules such as fluorescent dyes and their labeled antibodies.
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Fluorophore Photobleaching | Nikon’s MicroscopyU
https://www.microscopyu.com/references/fluorophore-photobleaching
Web ResultPhotobleaching (also termed fading) occurs when a fluorophore permanently loses the ability to fluoresce due to photon-induced chemical damage and covalent modification. Upon transition from an excited singlet state to the excited triplet state, fluorophores may interact with another molecule to produce irreversible covalent …
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Single Fluorophore Photobleaching | SpringerLink
https://link.springer.com/referenceworkentry/10.1007/978-3-642-16712-6_482
Web ResultDefinition. Photobleaching describes any process that leads to the permanent loss of fluorescence from light-emitting fluorescent species, including organic dye molecules, semiconductor particles such as quantum dots, and fluorescent proteins. As such processes terminate the flux of photons emanating from a fluorophore, …
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Photoblueing of organic dyes can cause artifacts in super ... - Nature
https://www.nature.com/articles/s41592-021-01061-2
Web ResultFeb 25, 2021 · Illumination of fluorophores can induce a loss of the ability to fluoresce, known as photobleaching.
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