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Addgene: CRISPR Guide
https://www.addgene.org/guides/crispr/
WEBThis guide will provide a basic understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR in your own research. Generating a Knockout Using CRISPR
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Addgene: CRISPR Plasmids and Resources
https://www.addgene.org/crispr/
WEBCRISPR Plasmids and Resources. Addgene is working with the leading scientists in the field to assemble the reagents and information you need to use CRISPR technology in your own lab. Browse plasmids below or check out our CRISPR resources on how to start using CRISPR in your lab.
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Addgene: CRISPR Pooled gRNA Libraries
https://www.addgene.org/crispr/libraries/
WEBCRISPR is a useful tool for genetic screening experiments, due to the relative ease of designing gRNAs and the ability to modify virtually any genetic locus. CRISPR pooled libraries consist of thousands of plasmids, each containing multiple gRNAs for …
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CRISPR History and Development for Genome Engineering - Addgene
https://www.addgene.org/crispr/history/
WEBCRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially discovered in the E. coli genome in 1987, but their function as a safeguard against bacteriophages was not elucidated until 2007.
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Addgene: CRISPR References and Information
https://www.addgene.org/crispr/reference/
WEBBrowse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.
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Addgene: CRISPR Plasmids - Tagging
https://www.addgene.org/crispr/tagging/
WEBThese CRISPR tagging methods allow for improved efficiency and throughput over traditional tagging methods. Several different tagging techniques, as well as the plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for more information about HDR/repair template design.
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CRISPR Topic Overview Page - Addgene
https://info.addgene.org/crispr-topic-page
WEBThe Class 2 Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) system is an adaptive immune system in bacteria. It's been modified for genome engineering and used for many other applications in biology. From base editing, to prime editing, to RNA editing, there's a lot of ways that CRISPR can be used. Learn more! …
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Addgene: CRISPR Plasmids - Mammalian Expression
https://www.addgene.org/crispr/mammalian/
WEBMammalian Expression. CRISPR Plasmids: Mammalian Expression. The following CRISPR plasmids have been designed for use in mammalian expression systems. Cut. Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence.
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How to Design Your gRNA for CRISPR Genome Editing - Addgene
https://blog.addgene.org/how-to-design-your-grna-for-crispr-genome-editing
WEBMay 3, 2017 · CRISPR technology has made it easier than ever both to engineer specific DNA edits and to perform functional screens to identify genes involved in a phenotype of interest. This blog post will discuss differences between these approaches, and provide updates on how best to design gRNAs.
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CRISPR 101: Homology Directed Repair - Addgene
https://blog.addgene.org/crispr-101-homology-directed-repair
WEBOur CRISPR 101 series was designed to help explain the basic principles driving CRISPRs and genome editing technology. Links: Zhang Lab’s CRISPR FAQs; CRISPR forum moderated by the Zhang lab; CRISPR Challenges: Standardization and HDR; CrispyCrunch; Optimizing Donor DNA for Enhanced CRISPR Genome Editing; General …
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