Keyword Analysis & Research: t4 dna ligase
Keyword Research: People who searched t4 dna ligase also searched
Search Results related to t4 dna ligase on Search Engine
-
T4 DNA Ligase | NEB
https://www.neb.com/en/products/m0202-t4-dna-ligase
Web ResultT4 DNA Ligase will ligate these substrates: dsDNA. Nicked DNA/RNA. Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some …
DA: 67 PA: 31 MOZ Rank: 72
-
Overview of T4 DNA Ligase: What it is, how it works ... - GoldBio
https://goldbio.com/articles/article/overview-of-T4-DNA-ligase
Web ResultT4 DNA ligase is an enzyme that fixes broken DNA and seals it – similar to super glue. This particular DNA ligase was isolated from bacteriophage T4. During DNA replication or recombination, a break or a ‘nick’ in …
DA: 19 PA: 33 MOZ Rank: 37
-
T4 DNA Ligase (5 U/μL) - Thermo Fisher Scientific
https://www.thermofisher.com/order/catalog/product/EL0011
Web ResultThermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on …
DA: 5 PA: 1 MOZ Rank: 54
-
Ligation Protocol with T4 DNA Ligase (M0202) | NEB
https://www.neb.com/en/protocols/0001/01/01/dna-ligation-with-t4-dna-ligase-m0202
Web ResultT4 DNA Ligase. This tutorial describes the use of the NEBioCalculator web tool to optimize the molar ratio between vector and insert DNA for use in a ligation reaction. Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the research community.
DA: 63 PA: 53 MOZ Rank: 75
-
Tips for Maximizing Ligation Efficiencies | NEB
https://www.neb.com/en/tools-and-resources/usage-guidelines/tips-for-maximizing-ligation-efficiencies
Web ResultNEB T4 DNA Ligase ( NEB #M0202) is the most extensively used ligase for cloning-based experiments. Traditionally, a ligation reaction (blunt or cohesive ends) using traditional T4 DNA Ligase involves incubation at 16°C using 0.1-1 µM DNA (5´ termini) in 1X T4 DNA Ligase Buffer.
DA: 85 PA: 98 MOZ Rank: 17
-
T4 DNA ligase structure reveals a prototypical ATP-dependent ligase
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212786/
Web ResultNov 2, 2018 · Thus, T4 DNA ligase represents a prototype of the larger eukaryotic and archaeal DNA ligases, with a uniquely evolved mode of protein interaction that may be important for efficient DNA replication. Go to: INTRODUCTION. DNA ligases seal breaks in the phosphodiester backbone of DNA ( 1–3 ).
DA: 44 PA: 94 MOZ Rank: 6
-
Ligation and Ligases - CSH Protocols
https://cshprotocols.cshlp.org/content/2019/8/pdb.top101352.full
Web ResultT4 DNA ligase, which is purified commercially from overproducing strains of E. coli ( Tait et al. 1980 ), has a Km of 6 × 10 −7 M for cohesive termini ( Sugino et al. 1977 ), 5 × 10 −5 M for blunt ends, and 1.9 × 10 −9 for nicks. The Km of the enzyme for ATP is ∼5 × 10 −5 M ( Weiss et al. 1968b ).
DA: 93 PA: 8 MOZ Rank: 97
-
Checking Your T4 DNA Ligase Activity - Thermo Fisher Scientific
https://www.thermofisher.com/us/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/checking-t4-dna-ligase-activity.html
Web ResultMolecular Biology Spotlight Articles ›. Checking Your T4 DNA Ligase Activity. Troubles with ligation? Check the activity of your T4 DNA Ligase with two easy-to-do control experiments.
DA: 10 PA: 49 MOZ Rank: 29
-
NEB - Ligation Protocol with T4 DNA Ligase (M0202) | NEB
https://www.neb.ca/neb/protocols.php?p=protocols/0001/01/01/dna-ligation-with-t4-dna-ligase-m0202
Web ResultT4 DNA Ligase. Videos. +. NEBioCalculator ® - Using the Ligation module. This tutorial describes the use of the NEBioCalculator web tool to optimize the molar ratio between vector and insert DNA for use in a ligation reaction.
DA: 6 PA: 17 MOZ Rank: 16
-
DNA ligase - Wikipedia
https://en.wikipedia.org/wiki/DNA_ligase
Web ResultThe T4 ligase is the most-commonly used in laboratory research. It can ligate either cohesive or blunt ends of DNA, oligonucleotides, as well as RNA and RNA-DNA hybrids, but not single-stranded nucleic acids. It can also ligate blunt-ended DNA with much greater efficiency than E. coli DNA ligase. Unlike E. coli DNA ligase, T4 …
DA: 75 PA: 12 MOZ Rank: 96