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Photobleaching - Wikipedia
https://en.wikipedia.org/wiki/Photobleaching
WEBIn optics, photobleaching (sometimes termed fading) is the photochemical alteration of a dye or a fluorophore molecule such that it is permanently unable to fluoresce. This is caused by cleaving of covalent bonds or non-specific reactions between the fluorophore and surrounding molecules.
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Photobleaching Principles | Thermo Fisher Scientific - US
https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-analysis-learning-center/molecular-probes-school-of-fluorescence/fluorescence-basics/fluorescence-fundamentals/photobleaching-principles.html
WEBWhat is photobleaching? A fluorophore can repeatedly undergo the fluorescence process—in theory, indefinitely. This is extremely useful, because it means that one fluorophore molecule can generate a signal multiple times.
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Photobleaching in Fluorescence Imaging - Thermo Fisher Scientific
https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-analysis-learning-center/molecular-probes-school-of-fluorescence/imaging-basics/protocols-troubleshooting/troubleshooting/photobleaching.html
WEBFluorescence that starts out bright but dims over time may be because the fluorophore is being degraded by light (photobleaching). Learn how to minimize it.
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Photobleaching in Fluorescence Microscopy - News-Medical.net
https://www.azolifesciences.com/article/Photobleaching-in-Fluorescence-Microscopy.aspx
WEBFeb 1, 2021 · Photobleaching in Fluorescence Microscopy. Download PDF Copy. By Dr. Surat P, Ph.D. Reviewed by Dr. Damien Jonas Wilson, MD. Photobleaching is the phenomenon when a fluorophore loses its fluorescence due to damage induced by light. This leads to loss of fluorescence and signal while imaging a sample. Real fluorescence …
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FRAP and Other Photobleaching Methods | SpringerLink
https://link.springer.com/referenceworkentry/10.1007/3-540-29623-9_4790
WEBPhotobleaching can reveal a protein's diffusional properties, movement between compartments within cells and life history. Photobleaching techniques depend on the availability of fluorescently labeled molecules such …
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How to Minimize Photobleaching - Enzo Life Sciences
https://www.enzolifesciences.com/science-center/technotes/2018/december/how-to-minimize-photobleaching/
WEBWhile photobleaching is unavoidable, many have employed practices that can mitigate the rate of degradation. One of the most common optimizations is simply reducing light intensity exposure to fluorophores.
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Fluorophore Photobleaching | Nikon’s MicroscopyU
https://www.microscopyu.com/references/fluorophore-photobleaching
WEBPhotobleaching (also termed fading) occurs when a fluorophore permanently loses the ability to fluoresce due to photon-induced chemical damage and covalent modification. Upon transition from an excited singlet state to the excited triplet state, fluorophores may interact with another molecule to produce irreversible covalent modifications. The ...
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Molecular Expressions Microscopy Primer: Fluorescence - Photobleaching
https://micro.magnet.fsu.edu/primer/java/fluorescence/photobleaching/
WEBSep 12, 2016 · Photobleaching. The phenomenon of photobleaching (also commonly referred to as fading) occurs when a fluorophore permanently loses the ability to fluoresce due to photon-induced chemical damage and covalent modification. Upon transition from an excited singlet state to the excited triplet state, fluorophores may interact with another …
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Single Fluorophore Photobleaching | SpringerLink
https://link.springer.com/referenceworkentry/10.1007/978-3-642-16712-6_482
WEBDefinition. Photobleaching describes any process that leads to the permanent loss of fluorescence from light-emitting fluorescent species, including organic dye molecules, semiconductor particles such as quantum dots, and fluorescent proteins. As such processes terminate the flux of photons emanating from a fluorophore, photobleaching serves as ...
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Photobleaching | SpringerLink
https://link.springer.com/chapter/10.1007/978-0-387-45524-2_39
WEBAbstract. Thanks to the wide variety of applications, fluorescence microscopy is now one of the most popular imaging techniques in biology (Weber, 1960; Lakowicz, 1999; Periasamy, 2001; Michalet et al., 2003; Tsien, 2003; Bastiens and Hell, 2004; Taroni and Valentini, 2004; Diaspro et al., 2005). Fluorescence microscopy utilizes fluorescently ...
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