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Ligase - Wikipedia
https://en.wikipedia.org/wiki/Ligase
In biochemistry, a ligase is an enzyme that can catalyze the joining ( ligation) of two molecules by forming a new chemical bond. This is typically via hydrolysis of a small pendant chemical group on one of the molecules, typically resulting in the formation of new C-O, C-S, or C-N bonds.
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Ligase - Definition and Examples - Biology Online Dictionary
https://www.biologyonline.com/dictionary/ligase
Jun 16, 2022 · Ligase is the class of enzyme that brings about the binding or joining of two molecules. It is, by definition in biology, the class of enzyme that catalyzes the binding or joining of two macromolecules by forming new bonds like C-O, C-N, and C-S. Ligase enzyme catalyzes the ligase reaction for, e.g. A-D + X A-X + d.
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DNA Ligase- Definition, Structure, Types, Functions - Microbe Notes
https://microbenotes.com/dna-ligase-structure-types-functions/
Aug 3, 2023 · DNA ligase is an enzyme that catalyzes the ligation reaction, ligation is the process of joining various types of DNA fragments. DNA ligase joins the DNA molecule covalently by catalyzing the formation of phosphodiester bonds between adjacent nucleotides.
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Restriction enzymes & DNA ligase (article) | Khan Academy
https://www.khanacademy.org/science/biology/biotech-dna-technology/dna-cloning-tutorial/a/restriction-enzymes-dna-ligase
If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase. DNA ligase seals the gap between the molecules, forming a single piece of DNA. Restriction enzymes and DNA ligase are often used to insert genes and other pieces of DNA into plasmids during DNA cloning.
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DNA ligase - Wikipedia
https://en.wikipedia.org/wiki/DNA_ligase
DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary ...
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Ligase | DNA replication, Enzymatic activity, Protein synthesis
https://www.britannica.com/science/ligase
Ligase, any one of a class of about 50 enzymes that catalyze reactions involving the conservation of chemical energy and provide a couple between energy-demanding synthetic processes and energy-yielding breakdown reactions. They catalyze the joining of two molecules, deriving the needed energy from.
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Ligase - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/ligase
Ligase, an enzyme that uses ATP to form bonds, is used in recombinant DNA cloning to join restriction endonuclease fragments that have annealed. The ligase commonly used is T4 DNA ligase, which was first isolated from E. coli that were infected with the lytic bacteriophage T4. The gene is encoded by the T4 genome and plays a role in the ...
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Ligase - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/ligase
Introduction. The term ligase is used for a variety of entirely unrelated enzymes. The common feature of all of them is their ability to covalently link two substrate molecules together. Here, the term will be used for DNA-ligases, which play a central role …
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DNA Ligase: Structure, Mechanism, and Function | Science
https://www.science.org/doi/10.1126/science.186.4166.790
DNA Ligase: Structure, Mechanism, and Function: The joining of DNA chains by DNA ligase is an essential component of DNA repair. replication, and recombination.
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DNA Ligases: Progress and Prospects - Journal of Biological Chemistry
https://www.jbc.org/article/S0021-9258(19)82019-7/fulltext
DNA ligases seal 5′-PO4 and 3′-OH polynucleotide ends via three nucleotidyl transfer steps involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are essential guardians of genomic integrity, and ligase dysfunction underlies …
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