| Keyword | CPC | PCC | Volume | Score | Length of keyword |
|---|---|---|---|---|---|
| fluorescent assay | 0.57 | 0.3 | 1539 | 82 | 17 |
| fluorescent | 0.96 | 1 | 6632 | 75 | 11 |
| assay | 0.65 | 1 | 2369 | 42 | 5 |
| Keyword | CPC | PCC | Volume | Score |
|---|---|---|---|---|
| fluorescent assay | 0.19 | 0.3 | 9863 | 99 |
https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/introduction-to-fluorescence-techniques.html
WebFluorescence Detection. Fluorescence Output of Fluorophores. Environmental Sensitivity of Fluorescence. Selected Books and Articles. Fluorescent probes enable researchers to detect particular components of complex biomolecular assemblies, such as live cells, with exquisite sensitivity and selectivity.
DA: 53 PA: 63 MOZ Rank: 21
https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/fluorescence-assay
WebFluorescence Assay. Ratiometric fluorescence assays depend on the measurement of the ratio of two signals, hence minimizing the influence of instrument and environmental conditions. From: Separation Science and Technology, 2022. Related terms: DNA; Lipid; Amyloid; Alpha-Synuclein; Thioflavin; Amino Acids; Enzyme; RNA; Mouse; Solution and …
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https://www.thermofisher.com/us/en/home/life-science/cell-analysis/fluorescence-microplate-assays.html
WebA variety of fluorescence microplate assays is available, from cell viability and proliferation assays to enzyme activity and protein quantification assays. These tools offer accurate and reliable measurements for kinetic and endpoint protocols across a wide range of …
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https://link.springer.com/protocol/10.1007/978-1-60327-545-3_7
WebJan 1, 2009 · Fluorescence-based assays are widely used in high-throughput screening due to their high sensitivity, diverse selection of fluorophores, ease of operation, and various readout modes. As a result, fluorescence-based assays have been applied to monitor a broad range of...
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7262998/
WebJan 2, 2019 · Fluorescence-based assays are one of the dominant measurement methods in HTS, because of their high sensitivity, good tolerance to interference, fast signaling speed, high versatility, simplicity, and non-destructive way of tracking or analyzing targets. 3–5 Rapid advancements in preparation of fluorescent materials with unique properties ...
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https://www.moleculardevices.com/technology/fluorescence
WebFluorescence is the property of some atoms and molecules to absorb light at a particular wavelength (the excitation: Ex) followed by a short-lived emission (Em) of light at a longer wavelength (Figure 2). The distance between the excitation and emission peaks is known as the Stokes shift and is fluorophore dependent (Figure 1).
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https://www.sciencedirect.com/topics/immunology-and-microbiology/fluorescence-assay
WebFluorescence Assay - an overview | ScienceDirect Topics. A fluorescence assay on live cells that is specifically designed to monitor flux relies on the use of a tandem monomeric red fluorescent protein (mRFP)–GFP-tagged LC3 (Klionsky et al., 2012). From: Autophagy: Cancer, Other Pathologies, Inflammation, Immunity, Infection, and Aging, 2014.
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https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/protein-assays/fluorescent-protein-assays.html
WebFluorescent Protein Assays. Fluorescence-based protein quantification detection methods provide superior sensitivity which means you use less of your protein samples for quantitation and have more samples available for your experiment. These fluorescence assays are also useful when colorimetric assays cannot be used due to interfering color …
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https://www.nature.com/articles/s41598-019-45356-z
WebJun 20, 2019 · Using fluorescence-based methods, we present a quick, safe, cost-effective, and real-time versatile nuclease assay, which uniquely studies nuclease enzyme kinetics. In conjunction with a...
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https://experiments.springernature.com/articles/10.1007/978-1-60327-545-3_7
Web(2009) Fluorescence-Based Assays. Authors: W. Frank An 1. Show more details. Citations: 6. PDF Full Text Related articles. Abstract. Fluorescence-based assays are widely used in high-throughput screening due to their high sensitivity, diverse selection of fluorophores, ease of operation, and various readout modes.
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764623/
WebDec 12, 2020 · The principle of fluorescence polarization immunoassay (FPIA) has been successfully applied to many analytical problems. Back in 1989, a review of FPIA developments listed 195 references [ 7 ]. The FPIA methodology has been successfully implemented in numerous commercial analytical systems.
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https://www.tecan.com/blog/how-to-develop-an-optimal-fluorescence-assay
WebFluorescence detection can give you the ability to develop assays with extreme sensitivity, high robustness and a broad dynamic range. Success involves addressing several challenges, such as the careful choice of excitation (Ex) and emission (Em) wavelengths and the selection of flexible and sensitive optics, as we will see here.
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9407474/
WebJul 27, 2022 · In summary, fluorescence assays have significant applications in both fundamental and clinical research and will facilitate the process of RNA-targeted new drug discovery, therefore deserving further development and updating. Keywords: RNA, chemical biology, fluorescent assays. 1. Introduction.
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https://www.corning.com/worldwide/en/products/life-sciences/resources/stories/at-the-bench/colorimetric-assay-luminescent-or-fluorescent-which-detection.html
WebColorimetric Assay, Luminescent or Fluorescent — Which Detection Tech Should You Select for Your ELISA? An ELISA (enzyme-linked immunosorbent assay) is a laboratory assay technique used to measure analytes such as peptides, hormones, and other antibodies in solution.
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https://pubmed.ncbi.nlm.nih.gov/19347618/
WebFluorescence-based assays are widely used in high-throughput screening due to their high sensitivity, diverse selection of fluorophores, ease of operation, and various readout modes.
DA: 30 PA: 28 MOZ Rank: 14
https://www.nature.com/articles/s42003-021-02881-w
WebNov 25, 2021 · Fluorescent cell barcoding (FCB) was developed as a cell-based multiplexing technique 4, 5, 6, 7. Distinct cell populations are labeled with unique signatures of amine-reactive fluorescent dyes...
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https://microbeonline.com/immunofluorescence-assay/
WebOct 18, 2023 · Immunofluorescence assay is a significant technique commonly used in immunology or molecular biology for detecting the presence and distribution of specific proteins or antigens. This process uses antibodies labeled with fluorescent molecules that bind to the target protein or antigen of interest.
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https://currentprotocols.onlinelibrary.wiley.com/doi/full/10.1002/9780470559277.ch090102
WebDec 1, 2009 · The technique of fluorescence polarization (FP) is based on the observation that when a fluorescently labeled molecule is excited by polarized light, it emits light with a degree of polarization that is inversely proportional to the rate of molecular rotation.
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https://bpsbioscience.com/fluorescence-polarization-assays-principles-applications
WebFP assays measure changes in light polarization that occur when a small fluorophore interacts with, or dissociates from, a much larger partner. Any small molecule which can be covalently labeled with a fluorophore and can form a complex with a larger partner is amenable to FP. Several controls are necessary to conduct a successful experiment:
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https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-quantitation/fluorescence-assays.html
WebFluorescence Assays. Regardless of throughput, we've got a fluorescence-based quantification kit for your DNA and RNA samples. Utilizing simple mix-and-read protocols and incorporating fluorescence-based detection reagents, these assays are designed for accuracy and convenience. Understand your sample’s purity and concentration.
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https://www.nature.com/articles/s41467-020-17962-3
WebAug 14, 2020 · A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice. Paris Roidos, Stephanie Sungalee, Salvatore Benfatto, Özdemirhan Serçin, Adrian M....
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https://link.springer.com/article/10.1007/s10895-024-03701-0
Web2 days ago · After incubation at room temperature for 10 min, the fluorescence spectrum of the resulting mixture was recorded using a fluorescence spectrophotometer under excitation of UV light at a wavelength of 380 nm. Assay of Methimazole Tablet Using the Enhanced-Fluorescent Nanoprobe Based on Cu NCs
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10255701/
WebMay 25, 2023 · There are a variety of fluorescence assays—which include immunoassays, foster resonance energy transfer (FRET), fluorescence correlation spectroscopy (FCS), and polarization—that are used for sensing applications [ 7, 8, 9, 10, 11, 12, 13 ].
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https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0012107
Web20 hours ago · Author summary Clonorchis sinensis is one of the most important foodborne zoonotic parasites, affecting over 15 million people worldwide. However, the low-intensity infection and the lack of instant detection methods remain the main inhibitory factors for controlling this disease. In this study, the specific CSTR1 antigen was combined with …
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https://chemrxiv.org/engage/chemrxiv/article-details/66266475418a5379b0628d73
Web2 days ago · This study reports the development of fluorometric assays for the detection and quantification of silyl hydrolase activity, using silicatein as a model enzyme. These assays employed a series of organosilane substrates containing either the mycophenolate or umbelliferone moieties, which become fluorescent upon hydrolysis of a scissile Si–O …
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https://pubs.acs.org/doi/10.1021/acs.analchem.4c00365
Web5 days ago · Here, a highly selective detection of miRNAs was developed that combined microsphere-enhanced fluorescence (MSEF) and solid-phase base-paired hybridization. The target miRNA could be accurately and quantitatively identified through the solid-phase hybridization assay on the surface of an optical microsphere, while the detected …
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https://www.thermofisher.com/order/catalog/product/23266
WebThe Thermo Scientific Pierce Fluorescent Protease Assay Kit measures total protease activity in samples, providing a means to assess the progress of protease isolation procedures or quantify protease contamination in biological samples.
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https://pubmed.ncbi.nlm.nih.gov/38656957/
WebFor qualitative analysis, the cut-off value of fluorescence for positive serum was 31.57 by testing 74 known negative human samples. The assay had no cross-reaction with other 9 parasite-infected sera, and could recognize the mixed …
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11030440/
WebApr 9, 2024 · A major limitation, however, is the lack of high-throughput assays to measure their activity. We developed the first fluorescence-based assay, DAB-APT, for measurement of APT activity using 1,2-diacetyl benzene, which forms fluorescent conjugates with putrescine, spermidine and spermine with fluorescence intensity …
DA: 68 PA: 8 MOZ Rank: 22
https://pubs.rsc.org/en/content/articlelanding/2024/cc/d4cc00238e
WebApr 18, 2024 · We integrate recombinase polymerase amplification (RPA) with CRISPR/Cas9-initiated nicking rolling circle amplification (CRISPR/Cas9-nRCA) for detecting Staphylococcus aureus. This approach utilizes a unique dimeric G-triplex structure, demonstrating firstly enhanced ThT fluorescence for target detection. Th
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