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293T cells for AAV production - Takara Bio
https://www.takarabio.com/products/gene-function/viral-transduction/adeno-associated-virus-(aav)/packaging-systems-and-cells/293t-cell-line
WEBOverview. 293T clone selected for high-titer AAV production. Provides high titers when combined with our AAVpro helper-free systems. More Information. Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications.
DA: 39 PA: 87 MOZ Rank: 100
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AAVpro® 293T Cell Line Protocol-At-A-Glance - Takara Bio
https://www.takarabio.com/documents/User%20Manual/AAVpro%20293T%20Cell%20Line%20Protocol/AAVpro%20293T%20Cell%20Line%20Protocol-At-A-Glance_090815.pdf
WEBAAVpro® 293T Cell Line Protocol-At-A-Glance Author: Clontech Laboratories, Inc. Subject: The AAVpro 293T Cell Line is a subclone of the transformed human embryonic kidney cell line, HEK 293, which supports high levels of viral protein expression. Keywords: Cat. No. 632273 Created Date: 9/8/2015 11:40:41 AM
DA: 99 PA: 15 MOZ Rank: 43
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Optimization of Large‐Scale Adeno‐Associated Virus (AAV) …
https://currentprotocols.onlinelibrary.wiley.com/doi/full/10.1002/cpz1.757
WEBMay 11, 2023 · Specifically, this protocol encompasses the following major steps: (1) passaging of AAVPro-293T cells; (2) plating of AAVPro-293T cells into T-175 tissue culture (TC) flasks; (3) transfection of AAVPro-293T cells for recombinant AAV production; and (4) harvest of recombinant AAV particles from AAVPro-293T cells.
DA: 28 PA: 83 MOZ Rank: 48
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AAVpro Extraction Solution - Takara Bio
https://www.takarabio.com/products/gene-function/viral-transduction/adeno-associated-virus-(aav)/packaging-systems-and-cells/extraction-solutions
WEBAAVpro Extraction Solution, a reagent set for the extraction of AAV particles from producer 293T cells, increases AAV yield by at least three-fold. AAV particle isolation is typically performed using freeze-thaw or sonication methods.
DA: 55 PA: 12 MOZ Rank: 68
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What cells are suitable for the production of AAV particles?
https://help.takarabio.com/36168/kb/article/109505/what-cells-are-suitable-for-the-production-of-aav-particles
WEBJun 27, 2023 · The AAVpro 293T Cell Line* or HEK 293T/17 cells (ATCC, CRL-11268) are recommended for preparation of high-titer AAV. The AAVpro 293T Cell Line is a subclone of the transformed human embryonic kidney cell line HEK 293, which is highly transfectable and supports high levels of viral protein expression. The cell line also constitutively expresses ...
DA: 69 PA: 59 MOZ Rank: 94
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OptimizationofLarge-Scale Adeno-AssociatedVirus(AAV) …
https://currentprotocols.onlinelibrary.wiley.com/doi/epdf/10.1002/cpz1.757
WEBSpecifically, this protocol encompasses the following major steps: (1) passaging of AAVPro-293T cells; (2) plating of AAVPro-293T cells into T-175 tissue culture (TC) flasks; (3) transfection of AAVPro-293T cells for recombinant AAV production; and (4) harvest of recombinant AAV particles from AAVPro-293T cells.
DA: 52 PA: 86 MOZ Rank: 63
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Protocol for evaluating the role of a gene in protecting mouse retinal
https://www.sciencedirect.com/science/article/pii/S2666166721006389
WEBDec 17, 2021 · This protocol includes the procedures for gene transfer through AAV intravitreal injection, induction of RGC injuries by NMDA-induced excitotoxicity or optic nerve crush, and retina immunohistochemistry to assess RGC survival. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021). Graphical abstract.
DA: 87 PA: 95 MOZ Rank: 46
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Feasibility of using NF1-GRD and AAV for gene replacement
https://www.nature.com/articles/s41434-019-0080-9.pdf
WEBplasmid (Stratagene) and AAVpro-293T cells (Clontech) were used. AAV production and purification pAAV-Rep-Cap, pscAAV, and pHelper plasmids in equal amount were transfected in AAVpro-293T...
DA: 66 PA: 42 MOZ Rank: 52
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Hypercompact adenine base editors based on a Cas12f variant
https://www.nature.com/articles/s41589-022-01077-5
WEBAug 1, 2022 · AAVpro 293T cells (Takara) were seeded at a density of 2 × 10 7 onto a triple flask with a dimension of 500 cm 2 (Thermo) before transfection. Vectors encoding base editor components, pHelper...
DA: 64 PA: 90 MOZ Rank: 51
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Proteomic Landscape of Adeno-Associated Virus (AAV) …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584267/
WEBOct 25, 2021 · 1. Introduction. Gene therapy using adeno-associated viral (AAV) vector delivery is a rapidly evolving field of the biotherapeutics industry. While the first recombinant AAV product, Glybera, was approved only in 2012, there are now over 150 clinical trials involving AAV registered on ClinicalTrials.gov [].However, despite this clinical activity, production of AAV-based therapeutics faces ...
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